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以刀鲚(Coilia nasus)为研究对象,运用荧光定量和相关生化测定方法,研究运输应激对下丘脑-垂体-肾间组织轴(HPI)基因表达和肝脏氧化指标的影响;同时采用浸泡的方式研究甘草甜素(glycyrrhizin,GL)对以上指标的调控作用。结果显示:运输后,脑中促肾上腺皮质激素释放激素(CRH)基因、阿黑皮素原(POMC)基因和硬骨鱼紧张肽(UI)基因的表达水平显著降低;头肾中皮质醇受体(GR)基因的表达水平运输2 h明显下降,4 h时表达水平最低,8 h显著升高。运输应激可以明显降低肝脏过氧化氢酶(CAT)的活性,显著提高肝脏丙二醛(MDA)的水平,而对谷胱甘肽过氧化物酶(GSH-PX)和脂质过氧化物(LPO)无显著影响。添加甘草甜素6 h后头肾GR基因的表达水平降低,脑中CRH和POMC基因的表达水平显著升高,但是脑中UI基因的表达水平无明显变化。此外,添加甘草甜素对肝脏CAT、MDA、GSH-PX和LPO无显著影响。以上结果表明,运输应激调控HPI轴的基因表达发生了不同程度的变化,而且降低了机体的抗氧化能力,使刀鲚处于氧化应激状态中。GL也可以影响HPI轴的基因表达,但不能有效缓解刀鲚的运输应激引发的氧化应激。
The effects of transport stress on the expression of hypothalamic-pituitary-renal axis (HPI) gene and hepatic oxidative stress were studied using fluorescence quantitative and related biochemical assays in Coilia nasus. Simultaneous immersion Methods Glycyrrhizin (GL) on the regulation of these indicators. The results showed that the expression of Corticotropin Releasing Hormone (CRH) gene, POMC gene and Urotensin (UI) gene in brain were significantly decreased after transport. The levels of cortisol in head kidney (GR) gene expression 2 h after transport decreased significantly, at 4 h the lowest level of expression, 8 h significantly increased. Transport stress could significantly reduce the activity of catalase (CAT) in the liver and significantly increase the level of liver malondialdehyde (MDA), while the activity of glutathione peroxidase (GSH-PX) and lipid peroxides (LPO) no significant effect. After adding glycyrrhizin for 6 h, the expression of GR gene in head kidney decreased, the expression of CRH and POMC gene in brain increased significantly, but there was no significant change in the expression of UI gene in brain. In addition, addition of glycyrrhizin had no significant effect on liver CAT, MDA, GSH-PX and LPO. The above results show that transport stress-regulated HPI axis gene expression changes in varying degrees, but also reduces the body’s antioxidant capacity, so that the knife 鲚 in the oxidative stress state. GL can also affect the gene expression of HPI axis, but can not effectively alleviate the oxidative stress caused by transport stress in the knife.