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目的建立阿立哌唑血药浓度的高效液相色谱内标法。方法色谱柱为Herpesil C18柱(250 mm×4.0 mm,5μm);流动相为2.85 mmol/L三乙胺-甲醇(20︰80),冰醋酸调pH值至7.5;流速为1.0 ml/min;柱温为40℃;检测波长为254 nm。结果阿立哌唑的线性范围为15~900μg/L,r=0.9966,平均方法学回收率95.0%(RSD=3.76%)。结论本法操作简单、准确,可以应用于日常临床监测阿立哌唑血药浓度。
Objective To establish a high performance liquid chromatography internal standard method of aripiprazole plasma concentration. Methods The column was Herpesil C18 (250 mm × 4.0 mm, 5 μm). The mobile phase was 2.85 mmol / L triethylamine-methanol (20:80). The pH was adjusted to 7.5 with glacial acetic acid. The flow rate was 1.0 ml / The column temperature was 40 ℃. The detection wavelength was 254 nm. Results Aripiprazole linear range of 15 ~ 900μg / L, r = 0.9966, the average recovery rate of 95.0% (RSD = 3.76%). Conclusion This method is simple and accurate and can be applied to routinely monitor aripiprazole plasma concentration.