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目的 研究碱性成纤维细胞生长因子 (b FGF)与转化生长因子 - β(TGF- β)在溃疡和增生性瘢痕组织中的表达特征以及与修复结果的关系。方法 2 1例标本包括体表慢性溃疡 8例、增生性瘢痕 8例和正常皮肤5例。用免疫组织化学法和常规病理技术确定两种生长因子在溃疡和增生性瘢痕的定位与表达量。结果 b FGF和TGF- β在正常皮肤中有少量表达 ,主要位于表皮基底细胞胞浆和细胞外基质。此外 ,b FGF还见于真皮毛细血管内皮细胞等。在溃疡组织 b FGF与 TGF- β表达量明显增加 ,其中 b FGF的阳性信号主要见于成纤维细胞和毛细血管内皮细胞 ,而 TGF- β则仅见于炎性细胞。在增生性瘢痕 TGF- β的表达为阴性 ,而 b FGF则仍呈现出较高的表达量。结论 在高浓度生长因子环境下创面修复延迟可能和生长因子与其受体结合障碍有关。研究结果还提示 b FGF参与了瘢痕发生的全过程 ,TGF- β则主要作用于瘢痕形成早期。
Objective To study the expression of basic fibroblast growth factor (b FGF) and transforming growth factor-β (TGF-β) in ulceration and hypertrophic scar tissue and its relationship with the repair outcome. Twenty-one specimens included 8 cases of chronic ulcer of body surface, 8 cases of hypertrophic scars and 5 cases of normal skin. Immunohistochemistry and routine pathological techniques were used to determine the localization and expression of both growth factors in ulcers and hypertrophic scars. Results b FGF and TGF-β were expressed in normal skin in small quantities, mainly in the epidermal basal cell cytoplasm and extracellular matrix. In addition, b FGF also found in dermal capillary endothelial cells and so on. In the ulcer tissue, the expression of bFGF and TGF-β increased significantly, of which the positive signal of bFGF was mainly found in fibroblasts and capillary endothelial cells, whereas TGF-β was only found in inflammatory cells. In hypertrophic scar TGF-β expression was negative, while b FGF still showed a higher expression level. Conclusion The delayed wound healing in high concentration of growth factor may be related to the disorder of growth factors and their receptor binding. The results also suggest that b FGF involved in the whole process of scarring, TGF-β mainly acts on the early scar formation.