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Objective to investigate the effects of antidiabetic drug metformin on proliferation and apoptosis in human hepatocellular carcinoma cell line Huh-7 cells.Methods Huh-7 cells were treated with metformin at different concentrations.Cell viability was determined by MTT assay.Cell apoptosis and CD133+expression rate were detected by flow cytometery (FCM) .Expressions of PTEN,Akt,p-Akt,Bcl-2,Bax proteins in the cells were measured by Western blot.The effect of metformin on the hepato-
Objective to investigate the effects of antidiabetic drug metformin on proliferation and apoptosis in human hepatocellular carcinoma cell line Huh-7 cells. Methods Huh-7 cells were treated with metformin at different concentrations. Cell viability was determined by MTT assay. Cell apoptosis and CD133 + Expressions of PTEN, Akt, p-Akt, Bcl-2, Bax proteins in the cells were measured by Western blot. The effect of metformin on the hepato-