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目的探讨潜在性转移生长因子结合蛋白-1(LTBP-1)mRNA和蛋白在子宫肌瘤组织中的表达及其与月经周期的关系。方法从手术标本中采集子宫肌瘤及同源正常子宫平滑肌组织。根据月经周期及组织病理学检查分为增生期、分泌期及萎缩期。采用实时荧光定量PCR、Western blot及免疫组织化学方法探查LTBP-1 mRNA和蛋白的表达。结果免疫组织化学染色显示LTBP-1在子宫肌瘤及正常子宫平滑肌组织的细胞外基质中均有较丰富的表达。LTBP-1mRNA和蛋白在增生期中的子宫肌瘤组织表达明显高于同源正常子宫平滑肌组织(P<0.05;P<0.01)。结论LTBP-1在增生期表达增高可能与子宫肌瘤的发生机制有关。
Objective To investigate the expression of potential metastatic growth factor binding protein-1 (LTBP-1) mRNA and protein in uterine fibroid tissue and its relationship with menstrual cycle. Methods Uterine fibroids and homologous normal uterine smooth muscle tissues were collected from surgical specimens. According to the menstrual cycle and histopathological examination divided into proliferative phase, secretory phase and atrophy. The expression of LTBP-1 mRNA and protein were detected by real-time fluorescence quantitative PCR, Western blot and immunohistochemistry. Results Immunohistochemical staining showed that LTBP-1 was abundantly expressed in the extracellular matrix of uterine fibroids and normal uterine smooth muscle tissues. The expression of LTBP-1 mRNA and protein in uterine fibroids in proliferative phase was significantly higher than that in normal uterine smooth muscle (P <0.05; P <0.01). Conclusion The increased expression of LTBP-1 in proliferative phase may be related to the pathogenesis of uterine fibroids.