论文部分内容阅读
为了观察灯盏花素对肝纤维化大鼠肝组织中TGF-β1和Smad3表达的影响,探讨灯盏花素抗大鼠肝纤维化的作用机制,方法:将大鼠32只随机分为对照组、模型组、水飞蓟宾组和灯盏花素组,采用皮下注射四氯化碳(CCl_4)造成大鼠肝纤维化模型,每周两次,连续8周。留取肝左叶行H.E.Masson染色,免疫组化方法检测各组TGF-β1和Smad3的表达,然后光镜下观察肝组织学变化和TGF-β1和Smad3表达量。结果与对照组比较,模型组TGF-β1和Smad3的表达明显增强(P<0.05),和模型组比较,灯盏花素可显著减少TGF-β1和Smad3的表达(P<0.05),而且肝纤维化减轻。表明灯盏花素可抑制大鼠肝纤维化模型中TGF-β1和Smad3的表达,从而发挥抗肝纤维化作用。
In order to observe the effect of Breviscapine on the expression of TGF-β1 and Smad3 in hepatic tissue of liver fibrosis in rats, and explore the mechanism of Breviscapine in inhibiting hepatic fibrosis in rats.Methods: 32 rats were randomly divided into control group, Model group, silibinin group and breviscapine group. The model of hepatic fibrosis was induced by subcutaneous injection of carbon tetrachloride (CCl 4) twice a week for 8 weeks. The liver left lobe was taken for H.E.Masson staining. The expressions of TGF-β1 and Smad3 in each group were detected by immunohistochemistry. The changes of liver histology and the expressions of TGF-β1 and Smad3 were observed under light microscope. Results Compared with the control group, the expression of TGF-β1 and Smad3 in the model group was significantly increased (P <0.05). Compared with the model group, breviscapine can significantly reduce the expression of TGF-β1 and Smad3 (P <0.05) Reduce. Breviscapine can inhibit the expression of TGF-β1 and Smad3 in rat hepatic fibrosis model and thus play an anti-hepatic fibrosis effect.