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采用香菇135双核体秋水仙碱(C22H25NO6)诱变及香菇K303单核体秋水仙碱诱变的菌株388B1与香菇JL-2双核体杂交(单×双)的方法,成功地获得了优良的诱变菌株(编号为K05)和杂交菌株(编号为K48)。经拮抗试验、酯酶同工酶、分子标记等多种方法鉴定和栽培试验证明,诱变、杂交菌株为有别于出发菌株和亲本菌株的新菌株;3年的栽培比较试验结果表明:新菌株比当地主栽香菇135、939高产,K05比135每筒平均增产28.5%,K48比939每筒平均增产10.7%,产量生物学统计显示增产显著;经济性状考评为优质,菌柄短、菇形圆整。该研究成果不仅有望满足菇农对香菇新菌株的需求,而且可为香菇育种提供新的途径。
An excellent lure was successfully obtained by using the mutagenesis of the rhubarb 135 double nucleocapsid (C22H25NO6) and the mutagenesis of the monocytogenetic colchicine 388B1 and the mushroom JL-2 dinucleus (Strain K05) and the hybrid strain (strain K48). Identification and cultivation experiments with antagonistic test, esterase isoenzyme and molecular marker proved that the mutagenic and hybrid strains were new strains different from the original strain and the parental strain. The results of three-year cultivation comparison showed that new The strains produced 135.939 higher yield than the local mushrooms, with an average yield of 28.5% per barrel for K05 to 135 and an average yield of 10.7% for K48 over 939. The biological yield statistics showed that the yield was significant. The economic traits were evaluated as high quality, Round shape. The results of this research are not only expected to meet the mushroom farmers demand for new strains of mushrooms, but also provide a new way for mushroom breeding.