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The current study was designed to examine the protective efficacy of DNA vaccines based on gp63 and Hsp70 against murine visceral leishmaniasis.Inbred BALB/c mice were immunized subcutaneously twice at an interval of three weeks with pcDNA3.1(+) encoding T cell epitopes of gp63 and Hsp70 individually and in combination.Animals were challenged intracardially with 10~7 promastigotes of Leishmania donovani 10 days post immunization and sacrificed 1,2 and 3 months post challenge.The immunized animals revealed a significant reduction(P < 0.05)in splenic and hepatic parasite burden as compared to the infected controls.Maximum reduction in parasite load(P < 0.05) was observed in animals treated with a combination of pcDNA/gp63 and pcDNA/Hsp70.These animals also showed heightened DTH response,increased IgG2a,elevated Th1 cytokines(IFN-γ and IL-2) and reduced IgG1 and IL-10 levels.Thus,mice immunized with the cocktail vaccine exhibited significantly greater protection in comparison to those immunized with individual antigens.
The current study was designed to examine the protective efficacy of DNA vaccines based on gp63 and Hsp70 against murine visceral leishmaniasis. Inbred BALB / c mice were immunized subcutaneously twice at an interval of three weeks with pcDNA3.1 (+) encoding T cell epitopes of gp63 and Hsp70 individually and in combination. Animal challenged intracardially with 10-7 promastigotes of Leishmania donovani 10 days post immunization and sacrificed 1, 2 and 3 months post challenge. The immunized animals revealed a significant reduction (P <0.05) in splenic and hepatic parasite burden as compared to the infected controls. Maximum reduction in parasite load (P <0.05) was observed in animals treated with a combination of pcDNA / gp63 and pcDNA / Hsp70. The animals also showed heightened DTH response, increased IgG2a, elevated Th1 cytokines (IFN-γ and IL-2) and reduced IgG1 and IL-10 levels.Thus, mice immunized with the cocktail vaccine loading significantly greater protection in comparison to that im munized with individual antigens.