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目的:评价hsa_circ_0025853在人神经母细胞瘤细胞(SK-N-SH细胞)氧糖剥夺/复糖复氧损伤(OGD/R)中的作用。方法:传代培养SK-N-SH细胞至对数生长期，采用随机数字表法分为4组(n n＝40)：对照组(C组)、OGD/R组、hsa_circ_0025853过表达组(E组)和hsa_circ_0025853过表达阴性对照组(EV组)。C组细胞在37 ℃、5%COn 2正常条件下培养，OGD/R组细胞铺于6孔板或96孔板待完全贴壁后氧糖剥夺16 h后复糖复氧。E组和EV组分别转染hsa_circ_0025853过表达载体或hsa_circ_0025853过表达阴性对照载体后制备OGD/R模型。于复糖复氧4和12 h时，采用qPCR法检测hsa_circ_0025853、线粒体动力相关蛋白1(Drp1)mRNA表达水平；Western blot法检测Drp1表达水平，CCK-8法检测细胞活力，流式细胞术检测细胞凋亡率。n 结果:与C组比较，OGD/R组复糖复氧后细胞活力降低，细胞凋亡率升高，Drp1及其mRNA表达上调，hsa_circ_0025853表达下调(n P<0.05)；与OGD/R组或EV组比较，E组复糖复氧后细胞活力升高，细胞凋亡率降低，Drp1表达下调，hsa_circ_0025853表达上调(n P0.05)。n 结论:hsa_circ_0025853表达下调可促进Drp1表达上调，诱发细胞凋亡，参与SK-N-SH细胞OGD/R的发生机制。“,”Objective:To evaluate the role of hsa_circ_0025853 in SK-N-SH cell oxygen-glucose deprivation-restoration (OGD/R) injury.Methods:SK-N-SH cells were cultured to the logarithmic phase and divided into 4 groups (n n=40 each) using the random number table method: control group (C group), OGD/R group, hsa_circ_0025853 overexpression group (E group) and hsa_circ_0025853 overexpression negative control group (EV group). The cells in C group were cultured under normal conditions of 37℃ and 5% COn 2, the cells in OGD/R group were placed in 6- or 96-well plates until they were completely attached to the wall, and then subjected to oxygen-glucose deprivation for 16 h, followed by restoration of oxygen-glucose.In E group and EV group, after cells were transfected with hsa_circ_0025853 overexpression vector and hsa_circ_0025853 overexpression negivative control vector, respectively, OGD/R model was established.At 4 and 12 h of restoration of oxygen-glucose, the expression of hsa_circ_0025853 and dynamin-related protein 1 (Drp1) mRNA was detected by quantitative polymerase chain reaction, the expression of Drp1 was detected using Western blot, and the cell viability and apoptosis rate of cells were detected using CCK-8 method and flow cytometry, respectively.n Results:Compared with C group, the cell viability was signifcantly decreased after restoration of oxygen-glucose, the apoptosis rate of cells was increased, the expression of Drp1 protein and mRNA was up-regulated, and the expression of hsa_circ_0025853 was down-regulated in OGD/R group (n P<0.05). Compared OGD/R group or EV group, the cell viability was signifcantly increased after restoration of oxygen-glucose, the apoptosis rate of cells was decreased, the expression of Drp1 protein and mRNA was down-regulated, and the expression of hsa_circ_0025853 was up-regulated (n P0.05).n Conclusion:The down-regulation of hsa_circ_0025853 expression can promote the up-regulation of Drp1 expression, induces cell apoptosis, and is involved in the mechanism of OGD/R in SK-N-SH cells.