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以4个非转基因大豆品种自贡冬豆、吉林小粒1号、中黄30和Williams 82以及2个抗草甘膦转基因大豆品系中作J9331和中作J9333为材料,利用大豆子叶节进行遗传转化,草甘膦作为筛选剂,以丛生芽诱导率、成活率和伸长率为考察指标,探索丛生芽诱导期、筛选期和伸长期的适宜草甘膦筛选浓度。结果表明,丛生芽诱导期、筛选期及伸长期的草甘膦适宜筛选浓度范围分别为100～160、220～240和5～10 mg.L-1,3个时期最适宜筛选浓度分别为60、240和5 mg.L-1。
Four non-genetically modified soybean varieties Zigong Dong Dou, Jilin Xiaoyu 1, Zhonghuang 30 and Williams 82, and 2 glyphosate resistant transgenic soybean lines J9331 and Zhongzhong J9333 were used as materials for genetic transformation with soybean cotyledonary node, Glyphosate was selected as the screening agent, and the inducement rate, survival rate and elongation rate of clustered shoots were taken as the index to explore the appropriate glyphosate screening concentration in inducing, screening and elongating stage. The results showed that the suitable screening concentration of glyphosate at the induction, screening and elongation stages of clustered shoots was 100 ~ 160, 220 ~ 240 and 5 ~ 10 mg, respectively, and the optimal screening concentration was 60 , 240 and 5 mg.L-1.