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将草菇菌株V23和耐低温诱变草菇菌株VH3的菌丝在4℃下处理不同时间,分别提取RNA,反转录获得cDNA作为模板,构建含Cor1基因和GPD内标基因片段的质粒作为标准品,采用荧光定量PCR技术研究Cor1基因在低温下的表达变化。结果表明草菇V23菌株的Cor1基因未经低温处理的表达量最大,低温处理后表达量持续下降,在6h达到最低值,8h开始该基因的表达量上升;而VH3菌株Cor1基因低温处理后表达量明显增加,2h达到最大值,4h下降,但6h开始上升,10h又下降。通过推测Cor1基因表达与草菇耐低温特性有相关性,为今后利用草菇自身的抗寒机制进行遗传改造提供理论依据。
The mycelia of V23 strain and Vp strain VH3 were treated at 4 ℃ for different time. RNA was extracted and cDNA was reverse transcribed to construct a plasmid containing Cor1 gene and GPD internal gene fragment Fluorescent quantitative PCR was used to study the expression changes of Cor1 gene at low temperature. The results showed that the expression of Cor1 gene of V23 strain was the highest without hypothermia treatment, and the expression level of Cor1 gene in V23 strain kept decreasing after 6 h, The amount increased significantly, reaching the maximum at 2h and decreasing at 4h, but began to increase at 6h and then decreased at 10h. By speculating that the expression of Cor1 is related to the low temperature resistance of straw mushroom, it provides a theoretical basis for genetic modification of straw mushroom with its own cold resistance mechanism in the future.