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目的通过建立实验性自身免疫性脑脊髓炎(EAE)动物模型,研究经腹腔诱导免疫耐受的方法及机理。方法腹腔内注射可溶性髓鞘碱性蛋白(MBP)或转输可溶性MBP致敏的腹腔抗原提呈细胞(MBP-APC),观察各组动物EAE的发病情况,检测各组动物迟发型超敏反应(DTH)和淋巴细胞体外增殖应答。结果腹腔内注射MBP及MBP-APC的动物发病率明显低于EAE组,临床症状减轻,由MBP引起的DTH和体外特异的MBP淋巴细胞增殖反应也明显降低。结论腹腔注射MBP或MBP-APC可引起粘膜耐受,抑制EAE的发生。
Objective To establish an animal model of experimental autoimmune encephalomyelitis (EAE) to study the method and mechanism of trans-abdominal induction of immune tolerance. Methods MBP was induced by intraperitoneal injection of MBP or MBP-APC sensitized by soluble MBP. The incidence of EAE in each group was observed, and the delayed type hypersensitivity (DTH) and lymphocyte proliferation in vitro response. Results The incidence of intraperitoneal injection of MBP and MBP-APC was significantly lower than that of EAE group. The clinical symptoms were relieved. The proliferative response of MBP-induced DTH and MBP-specific lymphocyte proliferation was also significantly decreased. Conclusion Intraperitoneal injection of MBP or MBP-APC can cause mucosal tolerance and inhibit the occurrence of EAE.