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目的通过对自发性高血压大鼠(SHR)颈上神经节形态学及其内神经肽酪氨酸(NPY)表达变化的研究,探讨NPY在高血压发生发展中的作用。方法随机选取成年SHR和WKY各20只,观测颈上交感神经节的位置、形状、大小及重量,采用Real-time PCR技术和免疫组织化学法,检测两组大鼠颈上神经节内NPY mRNA和蛋白的表达。结果与同周龄的WKY组大鼠相比较,SHR组大鼠血压明显升高(P<0.05);Real-time PCR和免疫组化结果显示:SHR颈上神经节内NPY mRNA和蛋白水平均较WKY增加(P<0.05)。结论 NPY在基因转录和蛋白表达等方面均较WKY上调,并参与高血压的形成。
Objective To investigate the changes of morphology and expression of neuropeptide tyrosine (NPY) in the superior cervical ganglia in spontaneously hypertensive rats (SHR) and to explore the role of NPY in the development of hypertension. Methods Twenty adult SHR and WKY were selected randomly to observe the location, shape, size and weight of the cervical sympathetic ganglia. Real-time PCR and immunohistochemistry were used to detect the expression of NPY mRNA And protein expression. Results Compared with WKY rats of the same age, the blood pressure of SHR group was significantly increased (P <0.05). Real-time PCR and immunohistochemistry results showed that both NPY mRNA and protein level in the superior cervical ganglion of SHR Than WKY increased (P <0.05). Conclusion NPY is up-regulated compared with WKY in gene transcription and protein expression, and is involved in the formation of hypertension.