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以‘小凤兰’根状茎为试材,研究了影响‘小凤兰’根状茎增殖、分化、生根壮苗的因素。结果表明:MS基本培养基、蔗糖30g/L、6-BA 2.0mg/L+NAA 0.5mg/L、活性炭0.5g/L有利于根状茎增殖,而添加20%椰汁和番茄汁40g/L会抑制根状茎增殖。1/2MS基本培养基、蔗糖30g/L、6-BA 2.0mg/L+NAA 0.2mg/L、20%椰汁、1 000lx光照强度有利于根状茎分化,不加活性炭有利于芽分化,但抑制根分化,而添加活性炭则抑制芽分化,促进苗分化。添加土豆泥60g/L显著促进试管苗生长,3~4cm高的苗在培养基1/2MS+6-BA 0.1mg/L+NAA0.5mg/L+蔗糖20g/L+土豆泥60g/L+卡拉粉8g/L+活性炭0.5g/L中培养40d即可移栽。
The experiment was conducted to study the effects of ’Xiaofenglan’ rhizome on the proliferation, differentiation, rooting and strong seedling growth of ’Xiaofenglan’ rhizome. The results showed that: MS medium, sucrose 30g / L, 6-BA 2.0mg / L + NAA 0.5mg / L, activated carbon 0.5g / L is conducive to the proliferation of rhizomes, while adding 20% coconut milk and tomato juice 40g / L will inhibit rhizome proliferation. 1/2 MS basic medium, sucrose 30 g / L, 6-BA 2.0 mg / L + NAA 0.2 mg / L, 20% coconut juice and 1000 lx light intensity were favorable for rhizome differentiation. But inhibited root differentiation, while adding activated carbon inhibited bud differentiation and promoted seedling differentiation. Add potato mashed 60g / L significantly promote the growth of test tube seedlings, 3 ~ 4cm high seedlings in the medium 1 / 2MS + 6-BA 0.1mg / L NAA0.5mg / L sucrose 20g / L mashed potatoes 60g / L + / L + activated carbon 0.5g / L in 40d can be transplanted.