论文部分内容阅读
目的对核酸检测法应用于血液标本内乙型肝炎(乙肝)病毒(HBV)检测的结果进行探究分析。方法 23465份无偿献血的血液标本,实施酶联免疫吸附法(ELISA)和核酸检测法(HBV-DNA),筛查HBV感染情况。对两种检测方法的结果进行分析。结果核酸检测法的特异性和敏感程度均优于酶联免疫吸附法(P<0.05)。核酸检测法漏诊情况少于酶联免疫吸附法,差异有统计学意义(P<0.05);核酸检测法窗口期为(20.9±4.2)d,少于酶联免疫吸附法(35.8±6.3)d(P<0.05)。结论将酶联免疫吸附法和核酸检测法联用可以减少漏诊情况,减少窗口期,此外核酸检测法的特异性和敏感程度很好,可以很好的检测乙肝病毒使得所输血液的安全性得到保障。
Objective To investigate the detection results of Hepatitis B virus (HBV) in blood samples by nucleic acid detection. Methods Totally 23465 blood samples donated by blood donation were tested for HBV infection by enzyme-linked immunosorbent assay (ELISA) and nucleic acid detection (HBV-DNA). The results of the two test methods were analyzed. Results The specificity and sensitivity of the nucleic acid detection method were better than those of enzyme linked immunosorbent assay (P <0.05). The number of misdiagnosis of nucleic acid detection was less than that of enzyme-linked immunosorbent assay (P <0.05). The window of nucleic acid detection was (20.9 ± 4.2) d, less than that of enzyme linked immunosorbent assay (35.8 ± 6.3) d (P <0.05). Conclusion Combination of enzyme-linked immunosorbent assay and nucleic acid detection can reduce the missed diagnosis and reduce the window period. In addition, the specificity and sensitivity of the nucleic acid detection method is very good, and the safety of the blood transfusion can be well detected by the hepatitis B virus Protection.