表达αβTcR 的T 淋巴细胞在胸腺内被分化为αβ~+与αβ~-,这种选择性作用有助于建立自身耐受性并保障成熟的CD_4~+、CD_8~+细胞群受自身MHC 约束;但有关γδT 细胞的发育所知不多。据此,本文开展这方面研究。将成年C_(57)BL/b(H-Z~b、TL~b)小鼠的CD_4~-、CD_8~-胸腺细胞和胸腺瘤BW5147融合后获得几株γδTcRT 杂交瘤。用杂交瘤生长抑制测定法鉴定一株转基因细胞特异的杂交瘤(KNb),它能识别TL~b 单型产物,后 T lymphocytes expressing αβTcR are differentiated into αβ ~ + and αβ ~ - in the thymus, and this selective effect contributes to the establishment of self-tolerance and protection of mature CD_4 ~ + CD_8 ~ + populations by their own MHC ; But little is known about the development of γδT cells. Accordingly, this article carries on the research in this aspect. Several γδTcRT hybridomas were obtained after fusion of CD_4 ~ -, CD_8 ~ -Thymocytes and thymoma BW5147 in adult C57BL / b (H-Z ~ b, TL ~ b) mice. A hybridoma-specific growth inhibition assay was used to identify a transgenic cell-specific hybridoma (KNb) that recognizes the TL-b haplotype