论文部分内容阅读
目的 探讨结直肠癌中脆性组氨酸三联体 (FHIT)基因蛋白表达状况及其与临床病理指标的可能关系。方法 采用兔抗人FHIT蛋白抗体和枸橼酸 微波 SP免疫组织化学方法检测 6 0例甲醛固定、石蜡包埋的结直肠癌蜡块标本中FHIT表达状况并分析其与组织学分级、Dukes分期以及 5年生存率的关系。结果 癌组织FHIT表达强度较正常黏膜高者为 2 1例 (35 0 % ) ,较正常黏膜低者为33例 (5 5 0 % ) ,与正常黏膜基本相等者为 6例 (10 0 % )。FHIT蛋白低表达癌在癌组织学分级中的分布为Ⅰ级癌 7/ 16 ,Ⅱ级癌 14/ 30 ,Ⅲ级癌 12 / 14,各级癌组间比较 ,差异有显著性 (P <0 0 5 )。FHIT蛋白低表达癌在Dukes分期中的分布为A期癌 5 / 11,B期癌 12 / 2 8,C期癌 16 / 2 1,已伴淋巴结转移组的C期癌与未转移组的A、B期癌比较 ,差异有显著性 (P <0 0 5 )。 39例获访病例中 ,FHIT蛋白低表达癌在 5年随访病例组中的分布为 5年生存组为 13/ 2 5 ,5年死亡组为 12 / 14,组间比较差异有显著性 (P <0 0 5 )。结论 结直肠癌FHIT表达状况可能与组织学分级、Dukes分期以及 5年生存率相关 ,提示FHIT表达降低可能对结直肠癌的演化和进展具有一定重要作用 ,并可能成为一个新的预后指标。
Objective To investigate the expression of FHIT gene in colorectal cancer and its possible relationship with clinicopathological parameters. Methods FHIT protein was detected by immunohistochemistry with rabbit anti-human FHIT protein and citric acid by microwave SP immunohistochemistry in 60 paraffin-embedded paraffin-embedded specimens of paraffin-embedded specimens of patients with colorectal cancer and analyzed their relationship with histological grade, Dukes stage and 5-year survival rate of the relationship. Results The expression of FHIT in cancerous tissue was 21 (35%) higher than that of normal mucosa, 33 (55.0%) lower than that of normal mucosa, 6 (10%) of normal mucosa. . The distributions of FHIT protein in the histological grade were grade Ⅰ 7/16, grade Ⅱ 14/13 and grade Ⅲ 12/12, with significant difference (P <0) 0 5). The distribution of FHIT protein in Dukes staging was 5/11 in stage A, 12/2 28 cases in stage B, 16/212 cases in stage C, and C in stage C with lymph node metastasis and A , B stage cancer, the difference was significant (P <0 05). In 39 cases, the distribution of FHIT protein low expression cancer in 5-year follow-up cases was 13/2 5 years in 5-year survival group and 12/14 in 5-year death group, the difference was significant (P <0 0 5). Conclusion The expression of FHIT in colorectal cancer may be related to the histological grade, Dukes stage and 5-year survival rate. It suggests that the decrease of FHIT expression may play an important role in the development and progression of colorectal cancer and may be a new prognostic indicator.