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目的初步探索艾滋病病毒1型(HIV-1)感染者血浆中可溶性程序性细胞死亡受体1(sPD-1)表达水平及临床意义。方法建立检测血浆sPD-1含量的双抗体夹心酶联免疫吸附试验(ELISA);收集26份尚未接受抗病毒治疗的HIV-1感染者和37份健康人外周血;利用双抗体ELISA检测血浆sPD-1表达水平,并分析其与临床参数的相关性。结果 HIV-1感染人群血浆sPD-1含量为(1147.81±375.7)pg/mL,显著高于健康人群(361.75±187.7)pg/mL(P<0.0001);HIV-1感染人群血浆sPD-1含量与外周血CD4+T淋巴细胞数量呈负相关(P=0.0229,r=-0.4444),与血浆病毒载量呈正相关关系(P=0.0089,r=0.5027)。结论 sPD-1在HIV-1的致病过程中可能起重要调控作用。
Objective To explore the plasma levels of soluble programmed cell death receptor 1 (sPD-1) in patients with HIV-1 infection and its clinical significance. Methods To establish a sandwich enzyme-linked immunosorbent assay (ELISA) for the determination of sPD-1 in plasma. 26 HIV-1-infected and 37 healthy volunteers were collected. The plasma sPD -1 expression level, and analyze its correlation with clinical parameters. Results The plasma level of sPD-1 in HIV-1 infected patients was (1147.81 ± 375.7) pg / mL, significantly higher than that in healthy subjects (361.75 ± 187.7 pg / mL, P <0.0001) (P = 0.0229, r = -0.4444), which was positively correlated with plasma viral load (P = 0.0089, r = 0.5027). Conclusion sPD-1 may play an important regulatory role in the pathogenesis of HIV-1.