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目的:对比不同分析方法对清喉咽合剂中黄芩苷含量测定的影响。方法:采用高效液相色谱法和正交函数光谱法对清喉咽合剂中黄芩苷进行含量测定。结果:正交函数光谱法黄芩苷在0.045~0.225 mg·ml-1范围内正交系数与浓度C之间呈现较好的线性关系,回归方程P2=0.091 7C-0.005 9(r=0.999 9);正交函数光谱法与HPLC法测定结果比较,差异无统计学意义(P>0.05)。结论:正交函数光谱法简便、快速、准确,检测成本低,可以对中药制剂中主要活性成分进行快速、准确的定量检测。
OBJECTIVE: To compare the effects of different analytical methods on the determination of baicalin in Qinghangyan Mixture. Methods: The contents of baicalin in Qinghangyan Mixture were determined by high performance liquid chromatography and orthogonal function spectroscopy. Results: The results showed that there was a good linear relationship between the orthogonal coefficient and the concentration C of baicalin in the range of 0.045 ~ 0.225 mg · ml-1, the regression equation P2 = 0.091 7C-0.005 9 (r = 0.999 9) There was no significant difference between the results of orthogonal function spectrometry and HPLC (P> 0.05). Conclusion: The orthogonal function spectrometry is simple, rapid, accurate and low in detection cost. It can detect the main active components in traditional Chinese medicine preparations rapidly and accurately.