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本研究采用核酸杂交技术、ＥＬＩＳＡ、~３Ｈ─ＴｄＲ掺入法及免疫荧光技术检测肿瘤患者及５００只带瘤ＢＡＬＢ／Ｃ小鼠Ｈｅ─Ｎｅ激光照射前后ＩＬ─２ｍＲＮＡ、ｓＩＬ─２Ｒ、ｍＩＬ─２Ｒ及ＩＬ─２活性的变化。研究结果表明，Ｈｅ─Ｎｅ激光照射具有促进ＩＬ─２基因表达的作用。激光照射组ＩＬ─２ｍＲＮＡ、ｍＩＬ─２Ｒ、ＩＬ─２活性均明显增加，而血清ｓＩＬ─２Ｒ减少，瘤块明显缩小，与非激光组比较（Ｐ＜０．０１），差异有高度显著性。此研究从基因水平上阐明Ｈｅ─Ｎｅ激光具有促进肿瘤患者内源性ＩＬ─２产生的作用，为肿瘤的治疗提供了一条新途径。
In this study, nucleic acid hybridization, ELISA, ~3H-TdR incorporation, and immunofluorescence were used to detect IL-2 mRNA, sIL-2R, and mIL-2R in tumor patients and before 500 He-Ne laser irradiation in tumor-bearing BALB/C mice. And changes in IL-2 activity. The results showed that He-Ne laser irradiation has the effect of promoting IL-2 gene expression. The activity of IL-2 mRNA, mIL-2R, and IL-2 in the laser irradiation group increased significantly, while the serum sIL-2R decreased, and the tumor mass decreased significantly compared with the non-laser group (P<0.01). The difference was highly significant. This study demonstrates from the gene level that He-Ne laser can promote the production of endogenous IL-2 in cancer patients, which provides a new way for tumor treatment.