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AIM:To detect the origin of hepatocellular carcinoma (HCC)recurring and attempt to propose a new recurrent mechanism.METHODS:Orthotopic liver allotransplantation was performedon male rats with HCC-induced by diethylnitrosamine usingfemale donors.Metastatic tumors in transplanted livers wereobtained.A DNA probe that exhibits specificity for the rat Ychromosome was generated by using a set of primers specificto murine srygene.In situ hybridization (ISH) for Y chromosomewas used to detected the origin of HCC recurring.Male HCCtissue was designed to be positive control.ISH on femaletissue and using non-labeled with DIG probe was thoughtto be negative control.RESULTS:Positive marks were seen through ISH for Ychromosome in recurrent tumor tissue and positive control.No signal was detected in both negative controls.CONCLUSION:Recurrent HCC after liver transplantationoriginated from disseminated tumor cells in recipients.Extrahepatic cells homing into liver may be a new HCCrecurrence mechanism.Likewise,it implicates that thismechanism is responsible for HCC recurring after hepatectomy.
AIM: To detect the origin of hepatocellular carcinoma (HCC) recurring and attempt to propose a new recurrent mechanism. METHODS: Orthotopic liver allotransplantation was performed on male rats with HCC-induced by diethylnitrosamine usingfemale donors. Metastatic tumors in transplanted livers wereobtained. A DNA probe that exhibits specificity for the rat Ychromosome was generated by using a set of primers specific to murine srygene. In situ hybridization (ISH) for Y chromosome was used to detect the origin of HCC recurring. Male HCCtissue was designed to be positive control .ISH on femaletissue and Using non-labeled with DIG probes was thoughtto be negative control .RESULTS: Positive marks were seen through ISH for Ychromosome in recurrent tumor tissue and positive control. No signal was detected in both negative controls. CONCLUSION: Recurrent HCC after liver transplantationoriginated from disseminated tumor cells in recipients. Extrahepatic cells homing into liver may be a new HCCrecurrence mechanism .Likewise, it implicates that this mechanism is responsible for HCC recurring after hepatectomy.