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目的:研究雷帕霉素对宫颈癌患者HeLa细胞侵袭转移抑制作用。方法:取宫颈癌患者HeLa细胞标本进行实验,空白组不加雷帕霉素,雷帕霉素组加入雷帕霉素30 nmol/L,分别培养48 h后,采用MTT法检测细胞活力(计算细胞生长抑制率)、Transwell法检测细胞迁移侵袭能力,以及采用Western blot法检测蛋白激酶B(Akt)和兔抗雷帕霉素靶蛋白(mTOR)的磷酸化水平以及兔抗基质金属蛋白酶-2(MMP-2)、兔抗基质金属蛋白酶-9(MMP-9)、波形蛋白(Vimentin)与钙黏蛋白(E-cadherin)表达水平。结果:雷帕霉素组患者宫颈癌HeLa细胞生长抑制率、E-cadherin表达率明显多于对照组(P<0.05),且宫颈癌HeLa细胞迁移和侵袭数量、Akt与mTOR磷酸化水平、MMP-2、MMP-9与Vimentin表达明显少于对照组(P<0.05)。结论:雷帕霉素能抑制宫颈癌HeLa细胞的侵袭转移,主要是通过Akt/mTOR信号通路发挥作用。
Objective: To study the inhibitory effect of rapamycin on the invasion and metastasis of HeLa cells in patients with cervical cancer. Methods: HeLa cell samples from cervical cancer patients were tested. In the blank group, no rapamycin was added, rapamycin (30 nmol / L) was added into the rapamycin group, and the cell viability was measured by MTT assay Cell growth inhibition rate). Transwell assay was used to detect cell migration and invasion. Western blot was used to detect phosphorylation of protein kinase B (Akt) and rabbit anti-rapamycin target protein (mTOR) (MMP-2), anti-MMP-9, Vimentin and E-cadherin were detected. Results: The growth inhibition rate and E-cadherin expression rate of cervical cancer HeLa cells in rapamycin group were significantly higher than those in control group (P <0.05), and the number of cervical cancer HeLa cell migration and invasion, Akt and mTOR phosphorylation, MMP -2, MMP-9 and Vimentin were significantly lower than those in the control group (P <0.05). Conclusion: Rapamycin can inhibit the invasion and metastasis of cervical cancer HeLa cells, mainly through the Akt / mTOR signaling pathway.