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用两种单克隆抗体（单抗）标记脐血造血祖细胞表面抗原（ＨＰＣＡ，ＣＤ＿（３４））用流式细胞仪分析，并比较两种单抗标记的细胞与体外培养的粒单细胞集落形成单位（ＣＦＵ－ＧＭ），红系爆式集落形成单位（ＢＦＵ－Ｅ）和混合集落形成单位的相关性，结果表明脐血有核细胞中，抗ＨＰＣＡ－２－ＦＩＴＣ阳性的细胞占１．０５１０．７２％，Ｔｕｋ３（纯抗体）阳性细胞占２．０６±１．２５％，差别显著（Ｐ＜０．０５），每μｌ脐血两种单抗标记的细胞分别为９６．５６±５６．６４和２３１．４０±１６３．９３（Ｐ＜０．０５），变异系数依次为５８．４７％和６８．４３％。尽管抗ＨＰＣＡ－２－ＦＩＴＣ阳性细胞与阳性细胞数量呈显著正相关，但前者与ＣＦＵ－ＧＭ，ＢＦＵ－Ｅ，ＣＦＵ－Ｍｉｘ以及ＣＦＵｓ（ＣＦＵ－ＧＭ＋ＢＦＵ－Ｅ＋ＣＦＵ－Ｍｉｘ）均呈正相关，而后者仅与ＣＦＵ－ＧＭ，ＣＦＵｓ呈正相关。研究结果提示在检测造血祖细胞时，用抗ＨＰＣＡ－２－ＦＩＴＣ代替可降低假阳性，获得较好的细胞与ＣＦＵ间的线性关系。
The surface antigen (HPCA, CD_ (34)) of cord blood hematopoietic progenitor cells was labeled with two kinds of monoclonal antibodies (mAb) and analyzed by flow cytometry. The two monoclonal antibody labeled cells were compared with the granulocyte single colonies cultured in vitro (CFU-GM), erythroid blast colony forming unit (BFU-E) and mixed colony forming units. The results showed that HPCA-2-FITC-positive cells accounted for 1 in cord blood nucleated cells. 0510.72%, Tuk3 (pure antibody) positive cells accounted for 2.06 ± 1.25%, the difference was significant (P <0.05), per mucosal cord blood two monoclonal antibody labeled cells were 96.56 ± 56 .64 and 231.40 ± 163.93 (P <0.05). The coefficients of variation were 58.47% and 68.43%, respectively. Although the positive number of positive anti-HPCA-2-FITC cells was positively correlated with the number of positive cells, the former was positively correlated with CFU-GM, BFU-E, CFU-Mix and CFUs (CFU-GM + BFU-E + CFU-Mix) And CFU-GM, CFUs was positively correlated. The results suggest that in the detection of hematopoietic progenitor cells, with anti-HPCA-2-FITC instead of false positives can be reduced to obtain a good linear relationship between cells and CFU.