通过紫外分光光度法,以异紫堇二酮的半合成转化产率为指标,详细考察反应时间、反应温度和投料比(异紫堇碱∶弗瑞米自由基)对转化产率的影响,确定异紫堇二酮的最佳合成条件:pH值为10的磷酸氢二钠溶液为反应介质、反应温度25℃、投料比(异紫堇碱∶弗瑞米自由基)=1∶2,反应时间12 h,通过氧化异紫堇碱可以半合成转化制备异紫堇二酮,产率可达到50.0%,并首次通过X-ray单晶衍射测定异紫堇二酮的化学结构。 The influence of reaction time, reaction temperature and feed ratio (iso-corydaldehyde: Frimide Radical) on the conversion yield was investigated by UV spectrophotometry with the semi-synthetic conversion yield of meta-violet dione as an index. The optimum synthesis conditions for the determination of different corydalone were as follows: the solution of disodium hydrogenphosphate (pH = 10) was used as the reaction medium, the reaction temperature was 25 ℃, the charge ratio (isoniazid: Frim) The reaction time was 12 h, and the iso-corydalone could be semi-synthetically transformed by oxidation of corynebacterium with the yield of 50.0%. The chemical structure of meta-corydione was determined by X-ray single crystal diffraction for the first time.