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建立了加工食品中天然色素胭脂树橙主要色素成分红木素、降红木素的液相色谱检测方法。采用氨水乙醇提取样品中的色素,石油醚除脂肪后,调节酸度使用二氯甲烷反提取目标色素,后采用C18色谱柱分离,流动相采用乙腈:2%乙酸(75∶25,V/V),458 nm波长光度测定。方法定量限为1.0 mg/kg,线性范围为1~100 mg/kg。在样品中做1.0,10,100 mg/kg三水平添加回收,降红木素、红木素回收率分别在83.2%~99.3%和84.6%~103%,RSD分别为0.90%~4.9%和1.7%~5.5%,并采用相对保留时间法来定性,吸光比值系数换算法来定量不同构型的红木素和降红木素。
The method of liquid chromatography for the determination of mahoganylin and mahoganylin as the main pigment of natural pigment bixin in processed foods was established. Ammonia ethanol was used to extract the pigment from the sample. After the petroleum ether was removed from the fat, the acidity was adjusted with methylene chloride to extract the target pigment. The residue was separated on a C18 column. The mobile phase consisted of acetonitrile: 2% acetic acid (75:25, V / V) , 458 nm wavelength photometry. The limit of quantification was 1.0 mg / kg with a linear range of 1 to 100 mg / kg. The recoveries of three samples were 1.0, 10, 100 mg / kg. The recoveries of redwood and mahogany were 83.2% -99.3% and 84.6% -103%, respectively. The RSDs were 0.90% -4.9% and 1.7% -5.5% %, And the relative retention time method to qualitative, absorbance ratio coefficient conversion method to quantify different configurations of redwood and redwood.