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目的:通过血管性痴呆大鼠模型,研究加减薯蓣丸对模型大鼠行为学及海马区脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)表达的影响,探讨加减薯蓣丸干预血管性痴呆的可能机制。方法:采用改良双侧颈总动脉阻断法,将大鼠双侧颈总动脉分次结扎并剪断,选取造模成功大鼠分为模型组和中药组,并设假手术组,每组20只。中药组予加减薯蓣丸10 g·kg-1·d-1,其余2组用等量生理盐水。连续灌胃8周后,各组大鼠进行Morris水迷宫实验(定位航行实验、空间探索实验);取海马组织,应用荧光定量PCR检测海马区BDNF mRNA表达水平。结果:与假手术组比较,模型组大鼠的逃避潜伏期明显延长、穿越平台次数减少、游动速度下降(P<0.05),海马组织BDNF mRNA表达水平明显降低(P<0.05);与模型组比较,中药组大鼠逃避潜伏期缩短,穿越平台次数和平台象限停留时间延长(P<0.05),中药组大鼠海马区BDNF mRNA表达明显升高(P<0.05)。结论:通过改良的双侧颈总动脉阻断法造模,可有效模拟血管性痴呆的行为学下降;加减薯蓣丸方可提高海马区下降的BDNF mRNA表达水平,这可能与影响神经突触可塑性作用有关。
Objective: To investigate the effects of yam pills on the behavior and the expression of brain-derived neurotrophic factor (BDNF) in hippocampus of rats with vascular dementia, Possible mechanisms of dementia. Methods: The bilateral common carotid arteries were ligated and cut by modified bilateral common carotid artery occlusion. The rats were divided into model group and traditional Chinese medicine group, and sham operation group only. The Chinese medicine group was treated by adding yam pills 10 g · kg-1 · d-1, the other two groups with the same amount of saline. After 8 weeks of continuous gavage, rats in each group were subjected to Morris water maze test (space navigation experiment, space exploration experiment); hippocampus tissue was taken, and the expression of BDNF mRNA in hippocampus was detected by real-time PCR. Results: Compared with the sham-operated group, the escape latency of model group was significantly longer, the number of passing through the platform decreased, the swimming speed decreased (P <0.05), and the level of BDNF mRNA in hippocampus significantly decreased (P <0.05) (P <0.05). The BDNF mRNA expression in the hippocampus of rats in TCM group was significantly increased (P <0.05) compared with the control group. Conclusion: The improved model of bilateral common carotid artery occlusion can effectively simulate the behavioral decline of vascular dementia. Adding or subtracting yam pills can increase the decreased expression of BDNF mRNA in hippocampus, which may be related to the influence of synapses Plasticity related.