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应用免疫荧光技术评价慢性化脓性中耳炎(COM)和分泌性中耳炎(SOM)耳渗出物中IgG和补体C_(3b)的细菌调理作用强度,并对渗出物的病原菌作定性和定量分析。渗出物样本来自16例COM20耳(年龄11个月～69岁)和13例SOM15耳(年龄8个月～13岁),C0M中耳脓性渗出物用无菌注射器经鼓膜穿孔处吸取,SOM中耳粘液样渗出物于全麻无菌下经鼓膜切开处吸取。渗出物样本分别作如下研究:①取血
Immunofluorescence was used to evaluate the intensity of bacterial conditioning of IgG and complement C_ (3b) in exudates of chronic suppurative otitis media (COM) and secretory otitis media (SOM). Qualitative and quantitative analyzes of the pathogen of exudate were performed. Exudate samples from 16 cases of COM20 ear (age 11 months to 69 years) and 13 cases of SOM15 ear (age 8 months to 13 years), C0M middle ear purulent exudate with a sterile syringe through the perforation of the eardrum , SOM middle ear mucoid exudate under general anesthesia by eardrum incision. Exudate samples were studied as follows: ① blood