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目的:观察茵栀黄注射液等28种中药制剂对大鼠CYP1A2的作用。方法:以非那西丁为探针,通过研究其体外转化率的变化评价受试中药制剂对大鼠CYP1A2的影响。结果:清开灵注射液、茵栀黄注射液、穿心莲注射液使非那西丁的体外转化率分别为(255.87±32.33)pmol.min-1.mg-1、(204.01±43.60)pmol.min-1.mg-1、(220.15±48.39)pmol.min-1.mg-1,显著低于对照组(432.68±69.71)pmol.min-1.mg-1(P<0.05)。3种药物的IC50分别为3.55,2.43,2.49 mL.(100 mL)-1。结论:清开灵注射液、茵栀黄注射液、穿心莲注射液体外对大鼠CYP1A2有抑制作用,且呈浓度依赖性。
Objective: To observe the effects of Yinzhihuang injection and other 28 kinds of Chinese medicine on rat CYP1A2. Methods: Phenacetin was used as a probe to evaluate the effects of the Chinese herbal preparations on CYP1A2 in rats by studying the changes in vitro conversion rate. Results: The conversion rates of phenacetin in Qingkailing injection, Yinzhihuang injection and Andrographis paniculata were (255.87 ± 32.33) pmol.min-1.mg-1 and (204.01 ± 43.60) pmol, respectively. min-1.mg-1, (220.15 ± 48.39) pmol.min-1.mg-1, significantly lower than the control group (432.68 ± 69.71) pmol.min-1.mg-1 (P <0.05). The IC50 of the three drugs were 3.55, 2.43 and 2.49 mL, respectively (100 mL) -1. Conclusion: Qingkailing Injection, Yinzhihuang Injection and Andrographis Paniculata Injection can inhibit CYP1A2 in vitro in a concentration-dependent manner.