为探索类胰岛素生长因子Ⅰ（ＩＧＦⅠ）基因转染许旺细胞的可能性，采用体外培养许旺细胞（ＳＣｓ），构建ＩＧＦⅠ逆转录病毒载体，利用基因转染技术将ＩＧＦⅠ基因转入ＳＣｓ，核酸分子杂交及细胞免疫组化检测ＳＣｓ 表达ＩＧＦⅠｍ ＲＮＡ及其蛋白水平，辅以正常ＳＣｓ作对照。结果：ｐＬＸＳＮＩＧＦⅠ转染的ＳＣｓ在体外相同培养条件下较正常ＳＣｓ 表达ＩＧＦⅠｍ ＲＮＡ 及其蛋白水平能力分别增强４８ 和３２ 倍，均有显著意义。研究表明，利用基因转染技术可将外源性ＩＧＦⅠ基因转入ＳＣｓ，提高ＳＣｓ中的ＩＧＦⅠ的含量和活性 In order to explore the possibility of transfecting Schwann cells with insulin-like growth factor-Ⅰ (IGF-Ⅰ) gene, we constructed the IGFⅠ retroviral vector by culturing Schwann cells (SCs) in vitro and using gene transfection technique to detect IGFⅠ Ⅰ gene into SCs, nucleic acid hybridization and cell immunohistochemistry detection of SCs IGF  Ⅰm RNA and its protein levels, supplemented with normal SCs as a control. Results: Compared with normal SCs, pLXSNIGFⅠ transfected SCs enhanced the expression of IGFⅠmRNA and its protein levels by 4. 8 and 32 times, respectively, in the same culture conditions. Studies have shown that the use of gene transfection technology can be exogenous IGF  Ⅰ gene transferred into SCs and improve the SCs IGF  Ⅰ content and activity