论文部分内容阅读
目的通过观察盐酸益母草碱(Leo)对药物流产后大鼠下丘脑-垂体-卵巢轴(HPOA)内分泌功能的影响,探讨Leo抑制子宫异常出血作用的机制。方法采用早孕大鼠灌服米非司酮(8.3 mg/kg)和米索前列醇(100μg/kg)造成不完全流产大鼠模型,用放射免疫分析法(RIA)观察其血清黄体生成激素(LH)、卵泡刺激素(FSH)水平的变化;免疫组化法检测下丘脑中促性腺激素释放激素(GnRH)的表达;RT-PCR检测子宫组织中孕酮受体(PR)、雌二醇受体(ERα、ERβ)mRNA的表达。结果 Leo明显提高大鼠血清中LH、FSH的水平和下丘脑中GnRH蛋白的表达,明显上调药流后大鼠子宫组织中ERα、ERβmRNA的表达(P<0.05或<0.01),而对PR mRNA没有明显影响(P>0.05)。结论 Leo能明显抑制药物流产后大鼠子宫异常出血,其机制可能与其调节或改善HPOA神经内分泌功能紊乱及子宫ERα、ERβ水平的变化密切相关。
Objective To observe the effect of Leo on the endocrine function of hypothalamus-pituitary-ovarian axis (HPOA) in rats after medical abortion and to explore the mechanism of Leo inhibiting uterine abnormal bleeding. Methods The pregnant rats were inflicted with mifepristone (8.3 mg / kg) and misoprostol (100 μg / kg) to induce incomplete abortion. The levels of serum luteinizing hormone (LH) and follicle stimulating hormone (FSH). The expression of gonadotropin-releasing hormone (GnRH) in the hypothalamus was detected by immunohistochemistry. The levels of progesterone receptor (PR), estradiol Receptor (ERα, ERβ) mRNA expression. Results Leo significantly increased the level of LH and FSH in serum and the expression of GnRH protein in hypothalamus, and significantly increased the expression of ERα and ERβmRNA (P <0.05 or <0.01) Significantly affected (P> 0.05). Conclusion Leo can significantly inhibit abnormal uterine bleeding after drug abortion in rats. The mechanism may be related to the regulation or improvement of neuroendocrine dysfunction of HPOA and the changes of ERα and ERβ in uterus.