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目的:研究固本防喘胶囊对哮喘动物模型气道嗜酸性粒细胞凋亡的影响,为临床应用提供理论依据。方法:体重200～250g雌性豚鼠50只,随机分为正常对照组、模型组、西药对照组、中药小剂量治疗组、中药大剂量治疗组,每组10只。正常对照组以生理盐水进行腹腔注射和雾化。其余4组采用卵蛋白诱导法建立哮喘模型;在第一次诱喘24h后,正常对照组及模型组予生理盐水,西药组予地塞米松片(1mg.kg-1)胃饲;中药小剂量组及中药大剂量组分别予固本防喘胶囊浓缩液(每mL含生药2g)小剂量(2.5g.kg-1)及大剂量(7.5g.kg-1)胃饲治疗10天。疗程结束后,处死动物,收集各组动物肺组织标本,TUNEL法检测肺组织中气道嗜酸性粒细胞凋亡情况。结果:中药大、小剂量组豚鼠气道嗜酸性细胞凋亡明显增加,与哮喘模型组比较有显著性差异(P<0.01),大剂量组与西药对照组相当(P>0.05)。结论:固本防喘胶囊能诱导或促进哮喘豚鼠气道EOS凋亡,从而减轻气道炎性细胞浸润,控制气道慢性炎症,达到防治哮喘的作用。
Objective: To study the effect of Guben Fangchuan Capsule on apoptosis of eosinophils in asthmatic animal model and provide theoretical basis for clinical application. Methods: Fifty female guinea pigs weighing 200-250 g were randomly divided into normal control group, model group, western medicine control group, Chinese medicine low-dose treatment group, and traditional Chinese medicine high-dose treatment group, 10 in each group. The normal control group was injected intraperitoneally with normal saline and atomized. The other 4 groups were induced by ovalbumin to establish an asthma model; 24 hours after the first asthma induction, the normal control group and model group received normal saline, and the western medicine group received dexamethasone tablets (1 mg.kg-1) for stomach feeding; The dose group and high-dose Chinese herbal medicine group were treated with low-dose (2.5g.kg-1) and large-dose (7.5g.kg-1) gavage for Guben Fangchuan Capsule Concentrate (2g per mL of crude drug) for 10 days. At the end of the treatment period, the animals were sacrificed and the lung tissue samples of each group were collected. The TUNEL method was used to detect the apoptosis of airway eosinophils in the lung tissue. RESULTS: The eosinophil apoptosis in guinea pigs in the large and small dose groups was significantly increased compared with the asthma model group (P<0.01). The high dose group was equivalent to the western medicine control group (P>0.05). Conclusion: Guben Fangchuan Capsule can induce or promote apoptosis of airway EOS in asthmatic guinea pigs, which can reduce airway inflammatory cell infiltration, control airway chronic inflammation, and prevent and treat asthma.