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【目的】利用在江西省南城县、宜黄县境内收集的118份野生毛花猕猴桃资源果实的20个表型性状,研究野生毛花猕猴桃初级核心种质的构建方法。【方法】采取逐步聚类,在30%取样比例下,以欧式距离为遗传距离,对3种取样方法(随机取样、偏离度取样和优先取样)、4种系统聚类方法(类平均法、离差平方和法、最长距离法和最短距离法)进行比较,以筛选出的最佳构建策略进一步比较不同取样比例(15%、20%、25%、30%、35%及40%)的构建效果,从而确定最适取样比例。【结果】(1)比较3种取样方法表明,采用优先取样法优于其他2种取样方法;(2)比较4种聚类方法表明,类平均法优于其他3种聚类方法;(3)最适取样比例为30%。【结论】通过主成分分析法验证表明,在30%取样比例下,采取欧式距离,利用类平均法进行逐步聚类,结合优先取样法构建的野生毛花猕猴桃初级核心种质能够最大程度地保留原始种质的遗传信息。
【Objective】 The objective of this study was to investigate the characteristics of primary core collection of wild kiwifruit kiwifruit using 20 phenotypic traits of 118 wild kiwifruit resources collected from Nancheng County, Yihuang County, Jiangxi Province. 【Method】 Three kinds of sampling methods (random sampling, sampling of deviance and preferential sampling), four kinds of systematic clustering methods (average sampling method, (15%, 20%, 25%, 30%, 35% and 40%) were compared by using the best construction strategy screened out by comparing with the square method, the square deviation method, the longest distance method and the shortest distance method. The construction effect, so as to determine the optimal sampling ratio. 【Results】 (1) Comparison of three sampling methods showed that sampling by priority was superior to the other two sampling methods. (2) Comparison of four clustering methods showed that the average of the three methods was superior to the other three clustering methods; (3) ) The optimum sampling ratio is 30%. 【Conclusion】 The results of principal component analysis showed that the Euclidean distances were obtained at the sampling rate of 30%, and the clustering was carried out by the method of cluster average. The primary core collection of wild Kiwifruit Genetic information of the original germplasm.