论文部分内容阅读
目的研究甘草Glycyrrhizae Radix et Rhizoma中的抗炎活性成分。方法采用大孔树脂、ODS、半制备液相等色谱手段进行分离纯化,并通过LC-MS、1H-NMR、13C-NMR等波谱技术进行结构鉴定。用细菌脂多糖(LPS)诱导小鼠巨噬细胞RAW264.7炎症模型对分离到的化合物进行抗炎活性筛选。结果从甘草中分离得到10个化合物,分别鉴定为甘草苷(1)、芹糖甘草苷(2)、异甘草苷(3)、芹糖异甘草苷(4)、sophoraisoflavone A(5)、粗毛甘草素F(6)、光甘草酮(7)、光甘草定(8)、甘草黄酮醇(9)和粗毛甘草素D(10)。结论化合物1、3、5、6、8和9对LPS诱导的RAW264.7细胞NO分泌有一定的抑制作用。其中化合物5、6和9抑制LPS诱导的RAW 264.7细胞NO分泌为首次报道。
Objective To study the anti-inflammatory active ingredients in Glycyrrhizae Radix et Rhizoma. Methods The macroporous resin, ODS and semi-preparative liquid were separated and purified. The structures were identified by LC-MS, 1H-NMR and 13C-NMR spectroscopy. Antibacterial activity of the isolated compounds was screened by bacterial lipopolysaccharide (LPS) induced RAW264.7 inflammatory model in mouse macrophages. Results Ten compounds were isolated from Glycyrrhiza uralensis Fisch. The compounds were identified as liquiritin (1), celery glycyrrhizin (2), isoliquiritin (3), glucomannan glycoside (4), sophoraisoflavone A Glycyrrhizin F (6), Glycyrrhizin (7), Glycyrrhizin (8), Glycyrrhiza flavonol (9) and Bromogaludin D (10). Conclusion Compounds 1, 3, 5, 6, 8 and 9 have some inhibitory effects on LPS-induced NO secretion in RAW264.7 cells. Among them, compounds 5, 6 and 9 inhibited the LPS-induced NO secretion of RAW 264.7 cells for the first time.