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目的：研究壳聚糖凝胶对精子活力的影响及其影响机制。方法将200例精液常规在正常范围的精液标本随机分为两组，每组各100例，对照组无任何处理，壳聚糖凝胶组加入壳聚糖凝胶。分别测定并比较两组中活动力为 a 级（快速向前运动）与 b 级（慢速或呆滞的前向运动）的精子百分率；形态学正常、头部缺陷、体部缺陷及尾部缺陷的精子数；精子顶体酶活性的定量值；精浆酸性磷酸酶、中性α-葡糖苷酶及锌的含量。结果壳聚糖凝胶组精子活动力较对照组显著降低，两组活动力为 a 级的精子百分率差异有统计学意义（ P ﹤0.05），活动力为 b 级的精子百分率差异无统计学意义（ P ﹥0.05）。壳聚糖凝胶组畸形精子数较对照组显著增加，两组形态学正常、头部缺陷尤其是尾部缺陷的精子数差异均有统计学意义（ P ﹤0.05），而中段缺陷的精子数差异无统计学意义（ P ﹥0.05）。壳聚糖凝胶组精子顶体酶活性的定量值较对照组降低，但差异无统计学意义（ P ﹥0.05）。壳聚糖凝胶组精浆酸性磷酸酶、中性α-葡糖苷酶及锌的含量均较对照组明显降低，差异均有统计学意义（ P ﹤0.05）。结论壳聚糖凝胶可显著降低精子的活动力并致精子畸形，同时引起精浆各生化指标不同程度地降低，因而具有显著的体外杀精作用，值得作为一种新型的外用避孕药物进一步研究与推广。“,”Objective To explore the effect of chitosan gel on sperm motility and the mechanism of this effect. Methods A total of 200 semen samples were randomly divided into two groups,control group( n = 100)without any treatment,chitosan gel was added to chitosan gel group(n = 100). Percentage of sperm motility of grade A(fast forward motion)and grade B(slow or sluggish),the number of normal,head de-fect,body defect and tail defect sperms,the activity of sperm acrosin enzyme,and the levels of seminal acid phosphatase,neutral α - glucosid and seminal zinc were determined and compared. Results The activity of sperm in chitosan gel group was lower than that in control group,there was statistical difference in the percentage of grade A sperms( P ﹤ 0. 05),but no difference in the percentage of grade B sperms( P ﹥ 0. 05)be-tween two groups. The number of deformity sperm in the chitosan gel group was significantly higher than that in the control group. There were sig-nificant differences in the number of normal,head defect and tail defect sperms( P ﹤ 0. 05),but not in the number of middle defect sperms( P﹥ 0. 05). The activity of sperm acrosin enzyme in chitosan gel group was lower than that in control group,but not statistically significant( P ﹥0. 05). The levels of seminal acid phosphatase,neutral α - glucosid and seminal zinc in chitosan gel group were lower than those in control group, with statistical significance(all P ﹤ 0. 05). Conclusion Chitosan gel could significantly reduce the sperm motility and biochemical parameters of seminal plasma,and make sperm deformity. Therefore,chitosan has significant killing sperm effect in vitro and,which was regarded as a new type of contraceptive drug for further research.