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目的探讨马鞍山地区结核病患者结核分枝杆菌基因分型成簇的影响因素。方法采用PCR及琼脂糖凝胶电泳技术,选取15个多位点可变数目串联重复序列分析(MVLA),对104株结核分枝杆菌临床分离株进行基因分型,同时对基因分型成簇的影响因素进行分析。结果 104株结核分枝杆菌菌株共分为38个不同的基因型,成簇基因型占83.65%(87/104),单一基因型占16.35%(17/104)。耐药菌株、不同性别和不同治疗患者在成簇和单一基因型的分布上差异无统计学意义(χ2=0.036,P=0.849),但在不同年龄之间差异有统计学意义(P<0.05)。结论马鞍山地区结核分枝杆菌基因型成簇率较高,提示结核分枝杆菌近期传播的风险较高,应加强对结核病患者的管理。
Objective To investigate the influencing factors of genotyping of Mycobacterium tuberculosis in patients with tuberculosis in Ma’anshan area. Methods PCR and agarose gel electrophoresis were used to screen 104 clinical isolates of Mycobacterium tuberculosis by genotyping 15 genotypes with variable number tandem repeat analysis (MVLA) The influencing factors were analyzed. Results 104 strains of Mycobacterium tuberculosis were divided into 38 different genotypes. The clustered genotypes accounted for 83.65% (87/104) and the single genotypes accounted for 16.35% (17/104). There were no statistically significant differences in the distribution of cluster and single genotypes among drug-resistant strains, patients of different genders and different treatments (χ2 = 0.036, P = 0.849), but the difference was statistically significant (P <0.05 ). Conclusion The high rate of cluster of Mycobacterium tuberculosis genotypes in Maanshan district suggests that M. tuberculosis is more likely to spread in the near future and management of TB patients should be strengthened.