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目的:观察牛鼻的软骨链蛋白(cartilage link protein,HA-CLP)在裸鼠肺移植瘤边界的指示情况,并对3种影像学方法显示肿瘤区域进行比较分析,为勾画肺肿瘤放疗靶区提供一定的实验依据。方法:建立能稳定表达荧光素酶基因的人肺癌细胞A549-luc2移植瘤裸鼠模型,将分离纯化的HA-CLP用99 TcmO4标记后进行SPECT显像,设立99 Tcm-MIBI对照组。同时进行荧光素酶(Luciferase)活体荧光显像和MR显像,分别取两组中同一只裸鼠的3种影像图像进行融合,比较肿瘤区域和边界情况。结果:99 Tcm-HA-CLP组和99 Tcm-MIBI组3种影像重叠率分别为(94±0.75)%和(93±1.15)%(n=10),差异无统计学意义,z=-0.680,P=0.496。在各不同时间点的L/B值,99 Tcm-HA-CLP组和99 Tcm-MIBI组的差异均无统计学意义,P>0.05。不同荧光值段的L/B值,除2×105外,其余各段两组差异均有统计学意义,99 Tcm-MIBI组的L/B值大多更高(P<0.005),但99 Tcm-HA-CLP组的L/B值随着荧光值的降低而降低,更具有线性关系。其中,两组指示肿瘤边界的L/B值即诊断阈值分别为1.82±0.04和2.05±0.04。99 Tcm-HA-CLP组的L/B值在2h达到高峰,为3.24±0.28;99 Tcm-MIBI组在4h达到峰值,为4.67±0.53。两者的峰值差异无统计学意义,z=-1.739,P=0.082。结论:HA-CLP通过SPECT显像能够对肺肿瘤的边界进行很好的界定,可为临床提供一定的依据。3种影像学方法对肿瘤边界的指示作用更加全面,可在一定程度上减少单一影像学方法的缺陷和误差。
OBJECTIVE: To observe the expression of bovine nasal cartilage link protein (HA-CLP) on the border of lung xenografts in nude mice and to compare and analyze the three tumor imaging areas in order to outline the radiotherapy target of lung tumors A certain experimental basis. Methods: The A549-luc2 xenografts in nude mice were established by transfecting A549-luc2 cells with stable luciferase gene. SPECT imaging was performed on the 99 TcmO4 labeled HA-CLP and 99 Tcm-MIBI control group was established. At the same time, Luciferase fluorescence imaging and MR imaging were performed. Three images of the same nude mouse in two groups were fused respectively to compare tumor area and border. Results The overlap rates of 99 Tcm-HA-CLP group and 99 Tcm-MIBI group were 94 ± 0.75% and 93 ± 1.15%, respectively (n = 10) 0.680, P = 0.496. There was no significant difference in L / B value between 99 Tcm-HA-CLP group and 99 Tcm-MIBI group at each time point (P> 0.05). The values of L / B in different fluorescence values ranged from 2 × 105 except for 99 Tcm-MIBI group (P <0.005) The L / B value of -HA-CLP group decreased with the decrease of fluorescence value, which was more linear. Among them, the L / B values of the two groups indicating the tumor border, that is, the diagnostic thresholds were 1.82 ± 0.04 and 2.05 ± 0.04.99, respectively. The L / B value of the T-HA-CLP group reached a peak at 3.24 ± 0.28 and 99 Tcm- The MIBI group peaked at 4h, which was 4.67 ± 0.53. The peak difference between the two was not statistically significant, z = -1.739, P = 0.082. CONCLUSION: The boundary of lung tumor can be well defined by SPECT imaging of HA-CLP, which can provide some evidences for clinic. The three imaging methods have a more comprehensive indication of the tumor boundary, which can reduce the defects and errors of the single imaging method to a certain extent.