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背景:bcl-2,c-myc基因在不同肿瘤中的表达和意义不尽相同。目的:探讨凋亡相关基因bcl-2和c-myc在人类大肠腺癌中的表达及其与预后的关系。设计:非随机对照的实验研究。地点、材料和干预:实验在解放军第四军医大学西京医院病理科完成。72例人类大肠腺癌选自西京医院外科手术及活检标本,5例正常人类结肠组织取自第四军医大学病理学教研室尸检组织(排除有结肠疾病)。运用HE染色和免疫组织化学方法对72例人类大肠腺癌及5例正常人类结肠组织进行研究,计算凋亡指数及免疫组化阳性率。主要观察指标:①不同分化程度的大肠癌凋亡指数。②大肠腺癌中Bcl-2及c-Myc的表达。③bcl-2和c-myc的关系。结果:大肠腺癌的细胞凋亡随肿瘤分化的不同,其差异有显著性意义(t=16.84,P<0.01),肿瘤分化程度越高,凋亡指数值越大。bcl-2和c-Myc在大肠腺癌中均具有较高程度的表达,两者的阳性率分别为61.1%和66.7%。Bcl-2与大肠腺癌的分化程度有关(χ2=11.29,P<0.05),肿瘤分化越低,其表达也就越低。c-myc在不同分化程度大肠腺癌的表达差异无显著性意义。Bcl-2和c-Myc在大肠腺癌组织中的阳性反应呈显著正相关(r=0.40,P<0.05)。结论:大肠腺癌细胞凋亡与肿瘤的恶性程度有关,bcl-2与c-myc均与大肠腺癌的细胞凋亡的调控有关,推测bcl-2能阻断c
Background: The expression and significance of bcl-2 and c-myc genes in different tumors are different. Objective: To investigate the expression of apoptosis-related genes bcl-2 and c-myc in human colorectal adenocarcinoma and its relationship with prognosis. Design: Non-randomized controlled experimental study. Location, Materials and Interventions: Experiments were performed at the Xijing Hospital of the Fourth Military Medical University of PLA. Seventy-two human colon adenocarcinomas were selected from surgical and biopsy specimens from Xijing Hospital and five normal human colon tissues were obtained from the autopsy (excluding colon disease) from the Department of Pathology, Fourth Military Medical University. 72 cases of human colorectal adenocarcinoma and 5 cases of normal human colonic tissue were studied by HE staining and immunohistochemistry. The apoptosis index and the positive rate of immunohistochemistry were calculated. MAIN OUTCOME MEASURES: ① Apoptosis index of colorectal cancer with different degree of differentiation. ② colorectal adenocarcinoma of Bcl-2 and c-Myc expression. ③ the relationship between bcl-2 and c-myc. Results: The apoptosis of colorectal adenocarcinoma was significantly different with tumor differentiation (t = 16.84, P <0.01). The higher the degree of tumor differentiation was, the greater the apoptosis index value was. The positive rates of bcl-2 and c-Myc in colorectal adenocarcinoma were 61.1% and 66.7% respectively. Bcl-2 was associated with the degree of differentiation of colorectal adenocarcinoma (χ2 = 11.29, P <0.05). The lower the tumor differentiation, the lower the expression of Bcl-2. The expression of c-myc in different degree of differentiation of colorectal adenocarcinoma had no significant difference. There was a significant positive correlation between Bcl-2 and c-Myc in colorectal adenocarcinoma (r = 0.40, P <0.05). Conclusion: The apoptosis of colorectal adenocarcinoma is related to the degree of malignancy of tumor. Both bcl-2 and c-myc are involved in the regulation of apoptosis of colorectal adenocarcinoma. It is speculated that bcl-2 can block c