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目的探讨有氧运动联合膳食控制对2型糖尿病(T2DM)大鼠血清visfatin水平的影响。方法雄性SD大鼠62只,随机抽取8只大鼠作为正常对照组(C),喂以标准普通饲料。其余54只SD大鼠在喂饲高糖高脂膳食的基础上腹腔注射小剂量STZ,建立T2DM动物模型。然后对T2DM大鼠进行运动锻炼或/和膳食控制。DM组大鼠继续喂饲高脂高糖饲料,不进行运动锻炼;运动锻炼采用每天进行60 min的无负重游泳运动,每周6次;膳食控制采用与DM组等量的标准饲料。13周后,检测各组大鼠FPG、TG、TC、FINS和Visfatin的含量。结果 (1)与C组相比,DM组大鼠FPG和血清Visfatin含量显著升高,FINS含量显著降低(P<0.01);通过双因素方差分析可知,有氧运动可以降低T2DM大鼠FPG和血清Visfatin含量(P<0.05),虽可使FINS升高,但无显著性差异;而膳食控制对降低T2DM大鼠FPG和血清Visfatin含量没有显著性影响(P>0.05),但可使FINS显著升高(P<0.05);有氧运动联合膳食控制可使T2DM大鼠FPG和血清Visfatin含量进一步降低,使FINS进一步升高,但无显著性交互作用(P>0.05)。(2)与C组相比,DM组血清TC和TG含量均显著升高(P<0.01);通过双因素方差分析可知,有氧运动或膳食控制均能显著地降低T2DM大鼠血TC含量(P<0.05),且具有显著的交互作用(P<0.05),可使血清TC含量进一步降低。有氧运动并不能使T2DM大鼠TG含量显著降低,饮食控制能显著降低T2DM大鼠TG含量(P<0.01),而且有氧运动联合饮食控制虽然可使T2DM大鼠的TG含量进一步降低,但无显著的交互作用(P>0.05)。结论 (1)T2DM大鼠血清visfatin水平显著升高,可能是T2DM发生发展过程中对胰岛素分泌下降,血糖、血脂水平升高的一种代偿机制。(2)有氧运动能够降低T2DM大鼠的血糖和Visfatin水平,增加胰岛β细胞分泌胰岛素的能力,对T2DM的治疗具有非常重要的作用;而单纯的膳食控制不能显著降低T2DM大鼠的FPG和Visfatin含量,且有氧运动联合膳食控制对降低T2DM大鼠FPG和Visfatin没有显著的交互作用。
Objective To investigate the effects of aerobic exercise combined with dietary control on serum visfatin levels in type 2 diabetic rats. Methods Sixty two male Sprague-Dawley rats were randomly divided into normal control group (C) and normal rats. The remaining 54 SD rats were given high glucose and high fat diet on the basis of low-dose intraperitoneal injection of STZ, T2DM animal model was established. T2DM rats are then exercised or / and diet controlled. Rats in DM group were fed with high-fat and high-sugar diet without exercise. Exercise-free exercise was carried out daily for 60 min without load, six times a week. Dietary control consisted of the same amount of standard feed as DM group. After 13 weeks, the content of FPG, TG, TC, FINS and Visfatin in each group were detected. Results (1) Compared with group C, FPG and serum Visfatin in DM group were significantly increased and FINS content was significantly decreased (P <0.01) .According to two-factor analysis of variance, aerobic exercise can reduce FPG and Serum Visfatin levels (P <0.05), although can make FINS increased, but no significant difference; and dietary control to reduce FPG and serum Visfatin levels in T2DM rats had no significant effect (P> 0.05), but could make FINS significantly (P <0.05). The combination of aerobic exercise and dietary control could further decrease the levels of FPG and Visfatin in T2DM rats, and further increase FINS, but no significant interaction (P> 0.05). (2) Compared with group C, serum TC and TG contents in DM group were significantly increased (P <0.01) .According to two-way analysis of variance, aerobic exercise or dietary control could significantly reduce blood TC level in T2DM rats (P <0.05), and had a significant interaction (P <0.05), which can further reduce the serum TC content. Aerobic exercise did not significantly reduce TG content in T2DM rats, diet control significantly reduced TG content in T2DM rats (P <0.01), and although aerobic exercise combined with diet control could further decrease TG content in T2DM rats, No significant interaction (P> 0.05). Conclusions (1) Serum visfatin levels in T2DM rats are significantly increased, which may be a compensatory mechanism for the decrease of insulin secretion, blood glucose and blood lipid levels during the development of T2DM. (2) Aerobic exercise can reduce the level of blood glucose and Visfatin in T2DM rats and increase the insulin secretion ability of pancreaticβcells, which plays a very important role in the treatment of T2DM. However, simple dietary control can not significantly reduce the FPG and Visfatin content, and diet combined with aerobic exercise to reduce T2DM rats FPG and Visfatin no significant interaction.