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遗传多样性分析为黧蒴栲优良品种的培育和利用提供技术依据。利用AFLP技术对39个黧蒴栲半同胞家系进行遗传多样性分析,结果显示利用8对引物共扩增出清晰条带761条,其中有多态性的条带有565条,多态率为74.14%,半同胞家系之间遗传多样性较高。黧蒴栲不同优树采集地之间遗传多样性较大,遗传多样性表现出较明显的地域性。各优树采集地内的遗传多样性表现不一致:湖南通道、福建明溪、广东平远优树采集地内遗传变异小;广西永福、融水、平果3地优树采集地内遗传变异较大,存在较大的遗传多样性;广西苍梧优树采集地内遗传变异最大。黧蒴栲出现优良半同胞家系的几率和遗传多样性密切相关,遗传多样性高的出现优良半同胞家系的几率大。出现优良家系的几率与家系所处的地理位置具有相关性,优良率与家系所处的纬度呈高度负相关,与经度为中度负相关。
Genetic diversity analysis provided the technical basis for the cultivation and utilization of fine varieties of Castanopsis ostreatus. AFLP technique was used to analyze the genetic diversity of 39 families of C. occidentalis. The results showed that 761 clear bands were amplified by 8 pairs of primers, of which 565 were polymorphic bands with polymorphism rate of 74.14%, higher genetic diversity among half-sib families. The genetic diversity among the different provenances was higher in the order of Gypsophila patens, and the genetic diversity showed more obvious regional characteristics. The genetic diversity of each tree was inconsistent: the genetic variation in the access sites of Hunan Channel, Mingxi of Fujian and Youyi of Pingyao of Guangdong was small; the variation of genetic diversity in the sampling sites of Youyou, Rongshui and Pingguo in Guangxi was larger, Larger genetic diversity; Guangxi Cangwu trees collected the largest genetic variation. The probability of occurrence of excellent half-sib families in C. occidentalis is closely related to the genetic diversity, and there is a high probability of emergence of an excellent half-sib family with high genetic diversity. There is a correlation between the probability of having a good family and the geographic location of the family. The rate of good and bad is highly negatively correlated with the latitude of the family and negatively correlated with the longitude.