论文部分内容阅读
目的探讨锰接触对大鼠肝脏功能和病理组织学的影响。方法SD大鼠36只随机分为3组,每组12只。高、低剂量染锰组分别给予含二氯化锰5.0g/L和0.5g/L的水溶液自由饮食,对照组给予蒸馏水。持续60天后,每组取9只动物应用荧光分光光度法测定各组动物肝脏匀浆的丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)活性和蛋白质含量;另3只动物作肝组织切片,HE法染色,光镜下检测肝组织病理学的变化。结果(1)与对照组相比,高、低剂量染锰组肝脏匀浆ALT、AST、ALP的活性明显升高,高剂量组为(60.79±9.88)、(49.77±8.36)和(49.43±8.88)U/ml,P<0.01;低剂量组为(47.35±8.11)、(37.31±6.77)和(32.34±4.53)U/ml,上述各项变化均呈现显著的量-效关系(P<0.05);而PRO含量各组间无显著差异(P>0.0)。(2)高、低剂量染锰组肝脏组织学损害主要表现为不同程度的肝小叶结构紊乱、肝细胞索松散、肝细胞脂肪变性和粒细胞浸润。结论锰接触对大鼠肝脏功能和组织学产生了明显的损害。
Objective To investigate the effects of manganese exposure on liver function and histopathology in rats. Methods Thirty - six SD rats were randomly divided into three groups of 12 rats. High-dose and low-dose groups of manganese were given free diet containing 5.0g / L of manganese chloride and 0.5g / L of water respectively. The control group was given distilled water. After 60 days, nine animals in each group were tested for their alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) by fluorescence spectrophotometry ) Activity and protein content. The other three animals were stained with HE staining, and pathological changes of liver tissues were detected by light microscope. Results (1) Compared with the control group, the activities of ALT, AST and ALP in the liver homogenates of the high and low dose Mn-Zn groups were significantly increased (60.79 ± 9.88), (49.77 ± 8.36) and (49.43 ± (47.35 ± 8.11), (37.31 ± 6.77) and (32.34 ± 4.53) U / ml, respectively. All these changes showed a significant dose-response relationship (P < 0.05). There was no significant difference in the content of PRO among all groups (P> 0.0). (2) The histological damage of liver in high and low dose of manganese dyed group mainly showed disorder of hepatic lobule structure, loose loosening of hepatocytes, steatosis of hepatocytes and infiltration of granulocytes. Conclusion Manganese exposure caused obvious damage to liver function and histology in rats.