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本文对中国广西壮族自治区京族和汉族共158名健康无关个体18个常染色体STR基因座遗传多态性进行调查,采用QIAampDNA Blood Midi试剂盒提取DNA,用TyperTM15plus试剂盒进行PCR扩增和检测,结果在18个STR基因座共检出350个等位基因和875种基因型,其分布符合Hardy-Weinberg平衡定律(P>0.05)。所调查的广西京族和汉族人群18个STR基因座均具有较好识别能力。
In this study, we investigated the genetic polymorphisms of 18 autosomal STR loci in 158 unrelated individuals in Jing nationality and Han nationality in Guangxi Zhuang Autonomous Region of China. DNA was extracted using QIAamp DNA Blood Midi Kit and PCR amplified with TyperTM15plus kit .Results A total of 350 alleles and 875 genotypes were detected in 18 STR loci. The distribution was in accordance with Hardy-Weinberg equilibrium (P> 0.05). The surveyed 18 STR loci in both Jing and Han populations in Guangxi showed good recognition ability.