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为建立一种诊断和预测胰岛素依赖型糖尿病（ＩＤＤＭ）的免疫学定量检测指标，改良了Ｐｅ－ｔｅｒｓｅｎ的谷氨酸脱羧酶自身抗体（ＧＡＤ－Ａｂ）放射配体分析法，以试管内转录和翻译的标记６５ｋＤ谷氨酸脱羧酶作抗原，以蛋白Ａ凝胶作为抗体沉淀剂，用液闪仪测ｃｐｍ值。对１５例初发ＩＤＤＭ和４０例对照的测定结果显示：ＩＤＤＭ组ｃｐｍ值显著高于对照组（Ｐ＜０．００１）；以对照组ｘ±２ｓ作为阳性标准，则ＩＤＤＭ组阳性率为６７％（１０／１５），对照组为２．５％（１／４０）。故改良的ＧＡＤ－Ａｂ放射配体分析法对初发ＩＤＤＭ诊断的特异性为９７．５％，敏感性为６７％。且操作简便，易于推广。
To establish an immunological quantitative detection index for the diagnosis and prediction of insulin-dependent diabetes mellitus (IDDM), Pe-tersen’s glutamate decarboxylase autoantibody (GAD-Ab) radioligand assay was modified for in vitro transcription and The translated marker 65kD glutamic acid decarboxylase as antigen, protein A gel as antibody precipitant, measured by liquid scintillation cpm value. The results of 15 patients with IDDM and 40 controls showed that the cpm values in IDDM group were significantly higher than those in control group (P <0.001), while in control group x ± 2s was the positive standard, the positive rate in IDDM group was 67% (10/15) in the control group and 2.5% (1/40) in the control group. Therefore, the modified GAD-Ab radioligand assay for the diagnosis of IDDM was 97.5% with a sensitivity of 67%. And easy to operate, easy to promote.