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[目的]监测广东省流感流行情况,为预防和控制流行奠定基础。[方法]用9~11 d龄鸡胚和(或)MDCK细胞分离病毒,用常量红细胞凝集抑制法(HI)鉴定;人血清流感抗体检测用微量半加敏HI。[结果]2005年共分离到流感病毒929株,A(H3N2)为优势株;2006年共分离到630株,A(H1N1)为优势株;2006年2月广州市发现首例H5N1人禽流感;一般人群的流感抗体,2005年A(H3N2)阳性率较高(67.1%),2006年A(H1N1)阳性率较高(67.1%),B(Yamagata)仅少数阳性。病毒抗原性分析B/广东/845/05、B/广东/869/05与国家2005年标准株B/Zhejiang/2/01抗原性有明显差别,抗原性漂移尤为显著。[结论]广东全年都有流感病人,并分离到流感病毒,分离率高峰期为4~7月。流感监测对预防流行有重要意义。
[Objective] To monitor the prevalence of influenza in Guangdong Province and lay the foundation for the prevention and control of epidemics. [Method] Virus was isolated from 9 ~ 11 d chicken embryos and (or) MDCK cells and identified by constant hemagglutination inhibition (HI) assay. Human serum influenza antibody was detected by semiquantitative HI. [Results] A total of 929 strains of influenza virus were isolated in 2005, with A (H3N2) being the dominant strain. 630 strains were isolated in 2006, with A (H1N1) being the predominant strain. The first H5N1 human bird flu was found in Guangzhou in February 2006 The prevalence of influenza A (H3N2) in the general population was high (67.1%) in 2005, while the positive rate of A (H1N1) was higher (67.1%) in 2006 and only a few positives in B (Yamagata). Antigen analysis of virus B / Guangdong / 845/05, B / Guangdong / 869/05 and the national standard strain of 2005 B / Zhejiang / 2/01 antigenic significant differences, antigenic drift is particularly significant. [Conclusion] Influenza patients were isolated in Guangdong throughout the year, and influenza virus was isolated. The peak period of isolation rate was from April to July. Influenza surveillance is of great importance in preventing epidemics.