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目的:探讨解毒化瘀药含药血清对白血病HL60/ADR细胞Survivin基因表达的影响。方法:应用半定量逆转录聚合酶链反应(RT-PCR)法检测解毒化瘀药含药血清处理后HL60/ADR细胞Survivin基因的表达,并与干扰素作对照。结果:解毒化瘀药含药血清能够降低HL60/ADR细胞Survivin基因的表达。结论:解毒化瘀药含药血清通过降低HL60/ADR细胞Survivin基因的表达,逆转白血病细胞耐药。
Objective: To investigate the effect of drug-containing serum of Jiedu Huayu drug on Survivin gene expression in leukemia HL60 / ADR cells. Methods: The expression of Survivin gene in HL60 / ADR cells was detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and compared with interferon. Results: The drug-containing serum of Jiedu Huayu drug can reduce the expression of Survivin gene in HL60 / ADR cells. Conclusion: The drug-containing serum of Jiedu Huayu drug can reverse the drug-resistance of leukemia cells by decreasing the expression of Survivin gene in HL60 / ADR cells.