一、色谱条件的选择体液成分十分复杂,加之结构与原形药十分相似的代谢产物,使体液药物浓度的测定较通常的药物测定困难得多,因此,在进行 HPLC 测定时色谱条件的选择极为重要。(一)色谱柱的选择色谱柱的选择实际上就是柱填料的选择。柱填料大致分为薄壳型和全多孔微粒两大类,后者应用普遍。以硅胶为基质的 First, the choice of chromatographic conditions Body composition is very complex, combined with the structure and prototype drugs are very similar metabolites, the determination of the concentration of bodily fluids than the usual drug determination is much more difficult, therefore, when the HPLC determination of the chromatographic conditions of choice is extremely important . (A) the choice of column Column selection is actually the choice of column packing. Column packing is roughly divided into two types of shell type and full porous particles, the latter is common. Silica gel-based