论文部分内容阅读
目的分析肿瘤患者血小板相关抗体产生规律及临床意义,探寻肿瘤患者临床血小板输注无效对策。方法先用ELISA方法QUIKSCREEN试剂筛查反复输注血小板3次以上肿瘤患者的HLA IgG抗体,再将初筛阳性样本用PAKPLUS试剂确定HLA、HPA抗体,同时观察抗体阳性患者临床症状并判断输注疗效。结果116份样本中筛查出HLA IgG抗体44例,抗体阳性率37.93%。初筛阳性样本经检测确认HLA抗体18例,HPA抗体8例,未能确认8例,其中HPA抗体及HLA抗体同时阳性10例。抗体阳性率与血小板输注次数成正相关(P<0.01),且与输注效果之间的相关性有统计学意义(P<0.01)。结论肿瘤患者血小板抗体阳性率与血小板输注次数相关,且与输注效果有关。
Objective To analyze the regularity and clinical significance of the production of platelet-associated antibodies in cancer patients and explore the clinical countermeasures of platelet transfusion in cancer patients. Methods The ELISA method of QUIKSCREEN was used to screen HLA IgG antibodies in patients with repeated transfusion of platelets for more than 3 times. Then positive samples were screened by PAKPLUS reagent for determination of HLA and HPA antibodies. Meanwhile, the clinical symptoms of patients with positive antibody were observed and the infusion efficacy . Results Of the 116 samples, 44 cases of HLA IgG were screened, the positive rate of which was 37.93%. Positive samples of the first screening test confirmed 18 cases of HLA antibodies, 8 cases of HPA antibodies, 8 cases failed to confirm, including HPA antibodies and HLA antibodies in 10 cases at the same time. The positive rate of antibody was positively correlated with the number of platelet transfusion (P <0.01), and the correlation between the antibody titer and the infusion effect was statistically significant (P <0.01). Conclusion The positive rate of platelet antibodies in patients with cancer is related to the number of platelet transfusions and is related to the infusion effect.