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为了解Fas配体 (FasL)在髓系白血病细胞中的表达水平及其诱导Fas+敏感细胞发生凋亡的功能 ,用流式细胞术检测 38例髓系白血病患者 (外周血白细胞 >10 0× 10 9/L)的白血病细胞在新鲜分离及IL 2和IFN γ刺激后表面膜FasL的表达水平 ,将白血病细胞 ( 1× 10 6/ml)与Jurkat细胞 ( 1× 10 5/ml)混合培养 2 4小时后 ,用DNA电泳和流式细胞术双标法检测Jurkat细胞发生凋亡的情况。结果表明 :白血病细胞表面FasL表达量 ( 3.5 9± 1.0 5 ) %高于正常人 ( 0 .36± 0 .16 ) % ,P <0 .0 0 1,经IL 2和IFN γ刺激后其表达量明显增加 ( 7.78± 3.4 0 ) % ,P <0 .0 1;白血病细胞刺激前后诱导Jurkat细胞发生的凋亡率分别为 ( 8.2 8± 1.6 1) % ,( 10 .73± 2 .16 ) % ,差异具有显著性意义。结论 :FasL在髓系白血病细胞表面高表达且对Fas+的敏感细胞具有杀伤功能 ,推测Fas/FasL途径可能在白血病的“免疫逃逸”中发挥作用
In order to understand the expression of Fas ligand (FasL) in myeloid leukemia cells and its role in inducing apoptosis of Fas + sensitive cells, flow cytometry was used to detect 38 myeloid leukemia patients (peripheral blood leukocytes> 10 × 10 10 9 / L) leukemic cells were isolated freshly and the expression of FasL on the surface of the membrane after stimulation with IL 2 and IFN γ. The leukemic cells (1 × 10 6 / ml) were mixed with Jurkat cells (1 × 10 5 / ml) for 2 Four hours later, the apoptosis of Jurkat cells was detected by DNA electrophoresis and flow cytometry double labeling. The results showed that the expression of FasL on the surface of leukemia cells was (3.59 ± 1.05)% higher than that of normal people (0.36 ± 0.16%), P <0.01, (7.78 ± 3.4)%, P <0.01. The apoptosis rates of Jurkat cells induced by leukemia cells were (8.28 ± 1.6 1)%, (10.73 ± 2.16) %, The difference is significant. Conclusion: FasL is overexpressed on the surface of myeloid leukemia cells and has cytotoxic activity on Fas + -dependent cells. It is speculated that Fas / FasL pathway may play a role in “immune escape” of leukemia