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COPT是目前国内外学者已公认的血吸虫病血清学诊断方法之一.为便该法更为完善、可行,提高虫卵得量,本研究对COPT抗原制备过程中的有关因素作一较系统的研究,结果提示,(1)在制备COPT抗原时,实验兔感染血吸虫尾蚴后所获虫卵量似主要决定于合抱虫体的数量,所用阳性钉螺每杯不宜少于50只.(2)每只实验兔感染1.000～1.500条尾蚴为宜.(3)无论制备冰冻还是热超声干卵,感染后45天剖杀实验兔收集虫卵,制备抗原较为适宜.(4)雌兔或雄兔对虫卵得量似並无影响,均适宜于制备COPT抗原的实验用兔.本文还对制备过程中的其他有关因素作了进一步的探讨。
COPT is one of the serological diagnosis methods of schistosomiasis recognized by domestic and foreign scholars at present.In order to make the method more complete and feasible and to improve the amount of egg production, The results showed that: (1) In the preparation of COPT antigen, experimental rabbit infected with Schistosoma japonicum coccidiosis after the amount of eggs mainly depends on the number of entrapping worms, positive snails per cup should not be less than 50. (2) Each Only experimental rabbit infected 1.000 ~ 1.500 cercariae is appropriate. (3) No matter whether the preparation of frozen or thermosonic dry eggs, 45 days after infection, the experimental rabbits were collected to collect the eggs to prepare antigens more appropriate. (4) The amount of eggs did not seem to influence, are suitable for the preparation of experimental rabbits rabbit COPT antigen.In this paper, the other relevant factors in the preparation process were further explored.