检测淋巴细胞表面抗原常用间接免疫荧光法(IFA),但它对细胞的数量和活性要求较高,因检测前后标本难以长期保存,所以,很难大规模收检标本;此外,该法需用昂贵的荧光显微镜。免疫组化技术能弥补以上不足,其中硷性磷酸酶-抗硷性磷酸酶复合物(简称APAAP)技术因具有众多的优点而日益受到重视。但常规抽静脉血制备单个核细胞涂片进行染色,采集标本较困难, Indirect immunofluorescence (IFA) is commonly used to detect lymphocyte surface antigens. However, it requires high quantity and activity of cells. Because of the difficulty of long-term preservation of samples before and after detection, large-scale collection of specimens is difficult. In addition, Expensive fluorescent microscope. Immunohistochemical techniques can make up for the above shortcomings, of which the alkaline phosphatase-alkaline phosphatase complex (APAAP) technology has drawn increasing attention for its numerous advantages. However, conventional venous blood prepared mononuclear cell smear staining, collection of specimens more difficult,