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目的 了解新城疫病毒HN基因增强肿瘤抗原诱发的抗肿瘤免疫反应的作用 ,并对其作用机制进行探讨。方法 将构建的HN基因 癌胚抗原 (CEA)cDNA共表达质粒 (pcD CEA HN)免疫小鼠 ,通过淋巴细胞增殖实验、NK细胞活性检测了解HN对抗CEA免疫反应的影响 ;并以免疫组化的手段追踪HN质粒表达产物在体内组织的分布 ,以及HN质粒对荷瘤小鼠肿瘤生长、抗肿瘤免疫反应的影响。结果 (1)共表达质粒pcD CEA HN免疫小鼠获得了较其他对照组强的淋巴细胞增殖指数及NK细胞活性。 (2 )HN质粒在肌肉、肿瘤组织有明显的表达。 (3)HN质粒对肿瘤生长具有一定的抑制作用 ,并能增强荷瘤小鼠的抗肿瘤免疫。结论 新城疫病毒HN基因对肿瘤抗原诱导的抗肿瘤免疫具有增强作用
Objective To understand the role of the newcastle disease virus HN gene in enhancing antitumor immune responses induced by tumor antigens and to explore its mechanism of action. Methods The mice were immunized with the constructed HN CEA cDNA co-expression plasmid (pcD CEA HN). The effects of HN on the CEA immune response were evaluated by lymphocyte proliferation assay and NK cell activity assay. Immunohistochemistry was also performed. The method used to track the distribution of HN plasmid expression products in vivo and the effect of HN plasmid on tumor growth and anti-tumor immune responses of tumor-bearing mice. Results (1) The mice co-expressing the plasmid pcD CEA HN were immunized to obtain a stronger lymphocyte proliferation index and NK cell activity than the other control groups. (2) HN plasmid has obvious expression in muscle and tumor tissues. (3) HN plasmid has a certain inhibitory effect on tumor growth and can enhance tumor-bearing mice’s anti-tumor immunity. Conclusion Newcastle disease virus HN gene enhances tumor antigen-induced anti-tumor immunity